Coding

Part:BBa_K1500993:Design

Designed by: Rivka Baker Keusch   Group: iGEM14_UChicago   (2014-10-17)


DnaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1979
    Illegal XhoI site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1068
    Illegal AgeI site found at 2022
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 901
    Illegal SapI.rc site found at 2785


Design Notes

Required site-directed mutagenisis


Source

DH5alpha

References